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Messi didn’t play for Argentina during the recent international break.
The 30-year-old was left out of the friendlies against Italy and Spain due to ‘muscle soreness’.
He returned to Barcelona earlier this week and has been checked over by the club’s doctor.
However he’s still not been given the all-clear to start in today's La Liga trip to Sevilla.
Barcelona head to the Ramon Sanchez Pizjuan stadium with an 11-point cushion at the summit of the table.
Messi could be left out if he’s not 100 per cent with half an eye sure to be on Wednesday’s Champions League clash with Roma.
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“Barcelona will decide tomorrow whether to play Lionel Messi against Sevilla”
Catalan newspaper Mundo Deportivo report it will be decided just hours before today's game if he plays against Sevilla.
Messi has been training with his team-mates but appears to behind Gerard Pique and Marc-Andre Ter Stegen in their rehab from knee injuries they picked up on international duty for Spain and Germany.
Messi is the top scorer in La Liga with 25 goals and has featured in all but one of Barca's league games this campaign, only sitting out the match against Malaga following the birth of his third son.
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Barca have failed to win on two of their last three visits to Sevilla's famously atmospheric Sanchez Pizjuan stadium, however, and will be keen to extend their 36-game unbeaten record in the league.
They are targeting the Liga record of 38 games unbeaten, held by Real Sociedad from the 1979/80 season.
Barca are still missing midfield anchor Sergio Busquets with a toe problem, while left back Lucas Digne is out for three weeks with a muscle injury sustained on international duty with France.
Sevilla host runaway Bundesliga leaders Bayern Munich in the first leg of their Champions League quarter-final tie on Tuesday and will be without suspended midfielder Pablo Sarabia against Barca as well as veteran Jesus Navas, who is injured.
Sevilla vs Barcelona gets underway at 7.45pm (UK time).